GSK-3β antibody | knockout validation | Cell Signaling Technology 9315

This is a knockout-validated antibody summary, based on the publications " Fine-Tuning of the RIG-I-Like Receptor/Interferon Regulatory Factor 3-Dependent Antiviral Innate Immune Response by the Glycogen Synthase Kinase 3/β-Catenin Pathway", as cited below [1], and "Isoform-selective decrease of glycogen synthase kinase-3-beta (GSK-3β) reduces synaptic tau phosphorylation, transcellular spreading, and aggregation" for western blot knockout validation (figure 2a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal

Company: Cell Signaling Technology

Antibody: GSK-3β

Catalog number: 9315

Summary: Rabbit monoclonal antibody raised against a synthetic peptide corresponding to a sequence within human GSK-3.

Supplier recommended for WB, IP and IHC.

Reacts with human, mouse, rat and monkey GSK-3β. Detects total GSK-3β.

Validation Method

Western blot

Sample

Mouse embryonic fibroblasts from wild-type, GSK-3β knockout and GSK-3α / GSK-3β double knockout animals.

Primary incubation

Anti-GSK-3β antibody.

Secondary incubation

HRP-conjugated secondary antibody (KPL).

Detection

West Lightning ECL kit (Perkin Elmer).

Figure

See Figure 1 from [1].

References
  1. Khan K, Dô F, Marineau A, Doyon P, Clément J, Woodgett J, et al. Fine-Tuning of the RIG-I-Like Receptor/Interferon Regulatory Factor 3-Dependent Antiviral Innate Immune Response by the Glycogen Synthase Kinase 3/β-Catenin Pathway. Mol Cell Biol. 2015;35:3029-43 pubmed publisher
  2. Amaral A, Perez Nievas B, Siao Tick Chong M, González Martínez A, Argente Escrig H, Rubio Guerra S, et al. Isoform-selective decrease of glycogen synthase kinase-3-beta (GSK-3β) reduces synaptic tau phosphorylation, transcellular spreading, and aggregation. iScience. 2021;24:102058 pubmed publisher