GSK-3β antibody | knockout validation | BD Biosciences 610201

This is a knockout-validated antibody summary, based on the publication "A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3α and GSK3β in Regulating Embryonic Stem Cell Fate", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

GSK-3β antibody | knockout validation | BD Biosciences 610201 figure 1
Figure 1. Western blot analysis of GSK3α and GSK3β in WT, Gsk3α−/− and Gsk3β−/− ESCs clones. Courtesy from the Authors [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Biosciences

Antibody: GSK-3β

Catalog number: 610201

Summary: Mouse monoclonal IgG1 against rat GSK-3β (aa. 1-160). Reacts with human, mouse, rat, dog and chicken. Suitable for western blot, bioimaging, immunoprecipitation and immunohistochemistry.

Validation Method

Western blot

Sample

WT, Gsk3α−/−, Gsk3β−/−, and Gsk3α/β DKO ESCs. Cells were lysed in ice-cold RIPA cell buffer (TEKNOVA) supplemented with protease inhibitors (Thermo Scientific) and phosphatase inhibitors (Roche).

Primary incubation

1:1,000 dilution.

Secondary incubation

HRP-conjugated secondary antibodies.

References
  1. Chen X, Wang R, Liu X, Wu Y, Zhou T, Yang Y, et al. A Chemical-Genetic Approach Reveals the Distinct Roles of GSK3α and GSK3β in Regulating Embryonic Stem Cell Fate.. Dev Cell. 2017;43:563-576.e4 pubmed publisher