This is a knockout-validated antibody summary, based on the publication "MLKL Activation Triggers NLRP3-Mediated Processing and Release of IL-1β Independently of Gasdermin-D", as cited below [1], and "Calpain drives pyroptotic vimentin cleavage, intermediate filament loss, and cell rupture that mediates immunostimulation" for western blot knockout validation (figure 1a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: GSDMDC1
Catalog number: sc-81868
Summary: Mouse monoclonal IgG1 (kappa light chain) against recombinant human GSDMDC1. Recommended for detection of human GSDMDC1 by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Western blot
Control and GSDMD-KO THP-1 cells.
Goat anti-mouse IgG-HRP (Santa Cruz Biotechnology, sc-2005).
ECL Western blotting substrate (Thermo Fisher Scientific, PI32209) and either standard autoradiography film (Pierce, Rockford, IL) or an electronic luminescence detection platform (ChemiDoc XRS+ system, 170-8265; Bio-Rad Laboratories, Hercules, CA).
Western blot of GSDMDCR THP-1 cells. Actin was used as a load control. Please see Supplemental Figure 2d in the article [1].
RIPK3 (Novus Biologicals, NBP2-24588), NLRP3 (Adipogen, AG-20B-0014) are also KO validated (Fig S1).
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