GSDMDC1 antibody | knockout examination | Santa Cruz sc-393581

This is a summary of antibody examined with a knockout sample, based on the publication "The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD). This antibody detects unspecific bands.

GSDMDC1 antibody | knockout examination | Santa Cruz sc-393581 figure 1
Figure 1. BMDMs of the indicated genotype treated as indicated and analyzed by immunoblot after 2 h or 3 h for GSDMD, showing the pro-form (pro) and cleaved (p30) GSDMD. This antibody detects unspecific bands. From [1].
Antibody information

Mouse monoclonal IgG2b (kappa light chain)

Company: Santa Cruz

Antibody: GSDMDC1

Catalog number: sc-393581

Summary: Mouse monoclonal IgG2b (kappa light chain) specific for an epitope mapping between amino acids 169-188 within an internal region of mouse GSDMDC1. Recommended for detection of GSDMDC1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

Bone marrow-derived macrophages (BMDMs) from WT and GSDMD-null mice.

References
  1. Chu L, Indramohan M, Ratsimandresy R, Gangopadhyay A, Morris E, Monack D, et al. The oxidized phospholipid oxPAPC protects from septic shock by targeting the non-canonical inflammasome in macrophages. Nat Commun. 2018;9:996 pubmed publisher