This is a knockout-validated antibody summary, based on the publication "Gasdermin D deficiency attenuates arthritis induced by traumatic injury but not autoantibody-assembled immune complexes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative images of specimens of Gsdmd+/+ and Gsdmd−/−

Rabbit monoclonal IgG

Company: Abcam

Antibody: GSDMD

Catalog number: ab219800

Summary: Rabbit monoclonal antibody against a recombinant fragment of GSDMD. Reacts with mouse and rat. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), and flow cytometry (intra). Unsuitable for immunocytochemistry/immunofluorescence.

Western blot | Immunohistochemistry

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Figure 2. mice stained with GSDMD antibody. Brown and dark red show specific staining. Scale bar = 250 μm. Gsdmd+/+ and Gsdmd-/- macrophages were primed with 100 ng/ml LPS or 30 ng/ml TNF-α for 3 h and treated with 15 μM nigericin for 1 hour. From [1].

WT and Gsdmd−/− mouse joint tissues (IHC) and macrophages (WB).

WB: 1:1,000 dilution overnight at 4 °C.

IHC: 1:200 dilution overnight at 4 °C.

WB: 1:5,000 dilution secondary goat anti-rabbit IgG (A21109, Thermo Fisher Scientific, Waltham, MA, USA) for 1h.

IHC: Biotinylated goat secondary antibody (Vector Laboratories, BA-9200) for 1h.

WB: Li-Cor Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA).

IHC: DAB reagent (Vector Laboratories, SK-4100) was used for the peroxidase-substrate reaction.