This is a knockout-validated antibody summary, based on the publication "Granzyme A From Cytotoxic Lymphocytes Cleaves GSDMB to Trigger Pyroptosis in Target Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Abcam
Antibody: GSDMB
Catalog number: ab215729
Summary: Rabbit monoclonal IgG against recombinant full length protein within human GSDMB. Reacts with human by western blot, immunoprecipitation and flow cytometry.
Western blot | Immunohistochemistry
WT and GSDMB-KO1 OE19 cell pellets.
IHC: 3% H2O2.
IHC: Slides were scanned with Pannoramic SCAN 150 (3DHISTECH, Budapest,
Hungary) using 20x objective magnification (Objective lens, Plan-Apochromat).
Effect of CRIPSR-Cas9 knockout of GSDMB expression on GZMA-induced pyroptosis in OE19 cells. Data in verifies the specificity of the monoclonal anti-GSDMB antibody used for IHC and ICC analyses using WT and GSDMB-KO1 OE19 cell pellets (scale bar, 100 μm), with the negative staining performed with secondary antibody only. Please see Figure S6b and S10 in the article [1].
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