GRIP1 antibody | knockout validation | Abcam ab10491
DOI
//dx.doi.org/10.13070/ko.en.7.2186
Date
2017-03-16

This is a knockout-validated antibody summary, based on the publication "The transcriptional coregulator GRIP1 controls macrophage polarization and metabolic homeostasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

GRIP1 antibody | knockout validation | Abcam ab10491 figure 1
Figure 1. Western blot of GRIP1 in Mx1-Cre−/−; GRIP1fl/fl (wild type (WT)) and Mx1-Cre+/+; GRIP1fl/fl (conditional knockout (cKO)) murine M(IL4) BMDM. Vinculin served as a loading control. From [1].
Antibody information

Rabbit polyclonal IgG

Company: Abcam

Antibody: GRIP1

Catalog number: ab10491

Summary: Rabbit polyclonal IgG against an a synthetic peptide corresponding to KAT13C/ NCOA2 (GRIP1) aa 700-750. Reacts with mouse and human, by western blot, immunoprecipitation and immunohistochemistry (paraffin). It is predicted to work with rat, rabbit, guinea pig, cow, dog, pig, chimpanzee, ferret, gorilla, and chinese hamster.

Validation Method

Western blot

Sample

Bone marrow-derived macrophages (BMDM) from control and GRIP1-cKO mice.

Primary incubation

1:3,000 dilution

References
  1. Coppo M, Chinenov Y, Sacta M, Rogatsky I. The transcriptional coregulator GRIP1 controls macrophage polarization and metabolic homeostasis. Nat Commun. 2016;7:12254 pubmed publisher