GREM1 / Gremlin‑1 antibody | knockout validation | LSbio LS-C125371

This is a knockout-validated antibody summary, based on the publication "Excessive mechanical loading promotes osteoarthritis through the gremlin-1-NF-κB pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

GREM1 / Gremlin‑1 antibody | knockout validation | LSbio LS-C125371 figure 1
Figure 1. Safranin O staining and immunofluorescence of Mmp13, Adamts5, IκBα, phosphorylated IκBα (dual Ser32/36), HIF-2α, and gremlin-1 proteins in mouse knee joints of Cntl and cKO mice 8 weeks after surgery. Scale bars, 50 µm. The percentage of positive cells in the immunofluorescence is shown below. n = 5 biologically independent experiments. From [1].
Antibody information

Mouse monoclonal IgG

Company: LSbio

Antibody: GREM1 / Gremlin‑1

Catalog number: LS-C125371

Summary: Mouse monoclonal IgG against partial recombinant human Gremlin-1 (75-185) with a GST tag. Reacts with human, mouse and rat. Suitable for western blot and ELISA.

Validation Method

Immunohistochemistry

Sample

Control and Gremlin-1 cKO mouse knee joints. Tissue samples were fixed in 4% paraformaldehyde, decalcified and embedded in paraffin.

Primary incubation

1:250 dilution.

Detection

Fluorescence microscope (BZ-X710, Keyence, Osaka, Japan).

Clone note

The same clone (4C2) is sold as LifeSpan Biosciences LS-C125371; Abnova H00026585-M04; MilliporeSigma WH0026585M4.

References
  1. Chang S, Mori D, Kobayashi H, Mori Y, Nakamoto H, Okada K, et al. Excessive mechanical loading promotes osteoarthritis through the gremlin-1-NF-κB pathway. Nat Commun. 2019;10:1442 pubmed publisher