This is a knockout-validated antibody summary, based on the publication "Grb2 and GRAP connect the B cell antigen receptor to Erk MAP kinase activation in human B cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of the indicated proteins in DG75 cells (wt), and variants lacking either Grb2 (Grb2-ko) or GRAP (GRAP-ko) or both (dko) following stimulation with 20µg/ml anti-IgM F(ab’)2 fragments for the indicated times. From [1].

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: GRB2

Catalog number: 05-372

Summary: Mouse monoclonal IgG1κ against recombinant GRB2/Ash. Reacts with bovine, human, hamster, mouse and rat. Suitable for western blot and immunohistochemistry.

Western blot

WT and Grb2-KO DG75 cells. Cells were lysed with lysis buffer composed of 50 mM Tris-HCl (pH 7.8), 137 mM NaCl, 0.5 mM EDTA, 1 mM sodium orthovanadate, 10% (w/v) glycerol, 1% (w/v) NP40 and a protease inhibitor cocktail (Sigma Aldrich, P2714).

HRPO-coupled secondary antibodies (SouthernBiotech).

The same clone (3F2) is sold as LifeSpan Biosciences LS-C179884; EMD Millipore 05-372.