This is a knockout-validated antibody summary, based on the publication "DHHC7 Palmitoylates Glucose Transporter 4 (Glut4) and Regulates Glut4 Membrane Translocation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgM (kappa light chain)
Company: Santa Cruz
Antibody: GODZ
Catalog number: sc-377378
Summary: Mouse monoclonal IgM (kappa light chain) specific for an epitope mapping between amino acids 297-327 within a C-terminal cytoplasmic domain of human GODZ (ZDHHC3 ). Recommended for detection of GODZ of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including and equine, canine, bovine and porcine.
Western blot
Primary adipocytes from epididymal tissue of wildtype and DHHC3 KO mice. Cells were washed twice with PBS and lysed with cell lysis buffer (20 mM Tris pH 7.6, 150 mM NaCl, 0.5 mM EDTA, 0.5 mM DTT, 10 mM, 1% Triton X-100 or 1% NP-40, 10% glycerol, protease and phosphatase inhibitors).
1:1,000 dilution.
Horseradish peroxidase-conjugated secondary antibody (Bio-Rad).
ECL detection system (Life Technology).
Western blotting of of primary adipocytes from epididymal tissue of wildtype, DHHC3 KO and DHHC7 KO mice. Please see Figure 5a in the article [1].
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