GM130 antibody | knockout validation | Abcam ab52649

This is a knockout-validated antibody summary, based on the publication "Globozoospermia and lack of acrosome formation in GM130-deficient mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

GM130 antibody | knockout validation | Abcam ab52649 figure 1
Figure 1. Localization of AP1 and GM130 in control and GM130-deficient germ cells. GM130 protein was only identified in the germ cells of the control testes (E, F red), but not in those of the GM130-/- testes. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: GM130

Catalog number: ab52649

Summary: Rabbit monoclonal IgG against a synthetic peptide within human GM130 (aa 1-100). Reacts with cow, dog, human, monkey, and African green monkey. Does not react with mouse or rat.

GM130 antibody | knockout validation | Abcam ab52649 figure 2
Figure 2. Western blot of the indicated proteins in control and GM130−/− mouse germ cells. From [1].
Validation Method

Western blot | Immunohistochemistry

Sample

Tissue extracts were prepared using a Dounce homogenizer in cold RIPA buffer (25 mM Tris-HCl, pH 7.6, 150 mM NaCl, 1%NP-40, 1% sodium deoxycholate, and 0.1% sodium dodecyl sulfate) supplemented with 1 mM phenylmethylsulfonyl fluoride and a protease inhibitor cocktail (Roche, Indianapolis, IN, USA).

Detection

The membrane was scanned using the ODYSSEY Sa Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA).

References
  1. Han F, Liu C, Zhang L, Zhou Y, Qin Y, Wang Y, et al. Globozoospermia and lack of acrosome formation in GM130-deficient mice. Cell Death Dis. 2017;8:e2532 pubmed publisher