This is a knockout-validated antibody summary, based on the publication "Glioma tumor suppressor candidate region gene 1 (GLTSCR1) and its paralog GLTSCR1-like form SWI/SNF chromatin remodeling subcomplexes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunoblot of GLTSCR1 in control and Gltscr1 knockout mESCs. B-actin was used as loading control. Courtesy from the Authors [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: GLTSCR1

Catalog number: sc-515086

Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 506-574 mapping within an internal region of human GLTSCR1. Recommended for detection of mouse, rat and human GLTSCR1. Suitable for western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

Control and Gltscr1 knockout mESCs.

5% bovine serum albumin (VWR, Batavia, IL) in PBS containing 0.1% Tween -20 (PBST) for 30 mins at room temperature.

Overnight at 4°C.

Goat anti-mouse secondary antibodies (LI-COR Biotechnology, Lincoln, NE) conjugated to IRDye 680 for 1 h at room temperature.

Odyssey Clx imager (LI -COR Biotechnology, Lincoln, NE).