This is a knockout-validated antibody summary, based on the publication "Blocking microglial activation of reactive astrocytes is neuroprotective in models of Alzheimer's disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a κ
Company: Santa Cruz
Antibody: GLP-1R
Catalog number: sc-390774
Summary: Mouse monoclonal antibody against amino acids 91-145 mapping within an N-terminal extracellular domain of human GLP-1R. Recommended for detection of GLP-1R of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
Control and GLP-1R KO mouse microglia.
3% BSA for 1 h at RT.
1:1,000 dilution overnight at 4 ℃.
HRP-conjugated secondary antibodies for 1 h at RT.
ECL Western Blotting Substrate (Promega Corporation, Madison, WI, USA).
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