This is a knockout-validated antibody summary, based on the publication "Selective targeting of HDAC1/2 elicits anticancer effects through Gli1 acetylation in preclinical models of SHH Medulloblastoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Gli1 and tubulin (loading control) in in WT and Gli1/2-KO MEF cells. From [1].

Mouse monoclonal IgG1

Company: Cell Signaling

Antibody: GLI1

Catalog number: 2643S

Summary: Mouse monoclonal IgG1 against a recombinant human GLI1 protein. Reacts with human by western blot and immunoprecipitation.

Western blot

WT and Gli1/2-KO MEF cells. Cells were lysed using denaturing SDS/UREA buffer (50 mM Tris, 2% SDS, 10% Glycerol, 10 mM Na4P2O7, 100 mM NaF, 6 M Urea, 10 mM EDTA).

5% milk in Tris buffered saline with 0.1% Tween20.

1:1,000 dilution.

Gli2 (# AF3635 R&D) 1:1000 is also KO validated in the same Figure.