This is a knockout-validated antibody summary, based on the publication "GIT1 protects traumatically injured spinal cord by prompting microvascular endothelial cells to clear myelin debris", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. The knockout and overexpression efficiencies of GIT1 in BMECs were confirmed by western blotting. From [1].

Mouse monoclonal IgG1

Company: Novus

Antibody: GIT1

Catalog number: NBP2-22423

Summary: Mouse monoclonal antibody against a fusion protein amino acids 375-770 (C-terminus) of rat GIT1. Reacts with human, mouse and rat. Suitable for western blot, immunocytochemistry (immunofluorescence), immunohistochemistry, and immunoprecipitation.

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Figure 2. Spinal cord at 7 days post-SCI: (i) representative H&E images in the lesion site from the GIT1 WT and KO groups. Bar, 100 μm. (ii) IF staining of GIT1 (green) and CD31 (red) in the lesion epicenter from the GIT1 WT and KO groups. Nuclei were stained using DAPI (blue). Scale bar, 100 μm. From [1].

Western blot | Immunohistochemistry

WT and GIT1 KO mouse spinal cord tissues and mouse brain microvascular endothelial cells (BMECs).

WB: 5% BSA.

IHC: 10% bovine serum albumin (BSA).

WB/IHC: at 4° C overnight.

WB: secondary antibody (Jackson ImmunoResearch, USA).

IHC: for 50 min under darkness at room temperature.

WB: enhanced chemiluminescence (ECL) reagents.

IHC: Fluorescence microscope (AXIO Vert.A1 and Imager A2, Germany).

The same clone (S39B-8) is sold as Novus Biologicals NBP2-22423; OriGene TA326533; MilliporeSigma SAB5200600; LifeSpan Biosciences LS-C209512, LS-C309120; StressMarq Biosciences SMC-413.