GGPS1 antibody | knockout validation | Santa Cruz sc-271679

This is a knockout-validated antibody summary, based on the publication "GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

GGPS1 antibody | knockout validation | Santa Cruz sc-271679 figure 1
Figure 1. Western blot analysis of Ggpps in PD12-14 oocytes. From [1].
Antibody information

Mouse monoclonal mouse IgG1 κ (kappa light chain)

Company: Santa Cruz

Antibody: GGPS1

Catalog number: sc-271679

Summary: Mouse monoclonal mouse IgG1 κ (kappa light chain) against amino acids 1-300 representing full length human GGPS1. Recommended for detection of human protein by western blot, immunoprecipitation, immunofluorescence and ELISA.

Validation Method

Western blot | Immunohistochemistry

Sample

Oocytes and ovaries from control and Ggpps knockout mice. IHC: ovaries were fixed in 4% paraformaldehyde, embedded in paraffin, and sectioned into 5 μm slices.

Blocking agent

IHC: Goat serum.

Primary incubation

IHC: 1:50 dilution overnight at 4°C.

Secondary incubation

IHC: Elite ABC kit and DAB substrate.

References
  1. Jiang C, Diao F, Sang Y, Xu N, Zhu R, Wang X, et al. GGPP-Mediated Protein Geranylgeranylation in Oocyte Is Essential for the Establishment of Oocyte-Granulosa Cell Communication and Primary-Secondary Follicle Transition in Mouse Ovary. PLoS Genet. 2017;13:e1006535 pubmed publisher