GFPT1 antibody | knockout validation | Abcam ab125069

This is a knockout-validated antibody summary, based on the publication "Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

GFPT1 antibody | knockout validation | Abcam ab125069 figure 1
Figure 1. Western blot of the indicated proteins in H1975 cells with or without GFPT1 overexpression or knockout. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: GFPT1

Catalog number: ab125069

Summary: Rabbit monoclonal antibody against a synthetic peptide within human GFPT1 (aa 600-700). Reacts with mouse, rat, and human. Suitable for western blot, immunoprecipitation and immunohistochemistry (paraffin). Unsuitable for flow cytometry or immunocytochemistry.

Validation Method

Western blot

Sample

WT and GFPT1-KO H1975 cells.

References
  1. Zhang X, Yu K, Ma L, Qian Z, Tian X, Miao Y, et al. Endogenous glutamate determines ferroptosis sensitivity via ADCY10-dependent YAP suppression in lung adenocarcinoma. Theranostics. 2021;11:5650-5674 pubmed publisher