This is a knockout-validated antibody summary, based on the publication "Oncogenic Role of Guanylate Binding Protein 1 in Human Prostate Cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting results of GBP1 protein expression in the control and GBP1 KO cells. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: GBP1

Catalog number: ab131255

Summary: Rabbit monoclonal IgG against a synthetic peptide within human GBP1 (aa 550-650). Suitable for western blot, immunohistochemistry (paraffin), and immunocytochemistry/immunofluorescence. Unsuitable for flow cytometry or immunoprecipitation.

Western blot

Control and GBP1-KO human prostate cancer cell lines DU145 and PC3.

5% non-fat dry milk in Tris-buffered saline with Tween-20 (TBST) for 2 h at room temperature.

1:500 dilution.

ECL Plus kit (Thermo Scientific, USA).