This is a knockout-validated antibody summary, based on the publication "Glucosylceramidase Maintains Influenza Virus Infection by Regulating Endocytosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Complete loss of GBA protein expression was confirmed by Western blot analysis of lysates of both HEK 293 and A549 cell colonies. From [1].

Mouse monoclonal IgG2a

Company: Abcam

Antibody: GBA

Catalog number: ab55080

Summary: Mouse monoclonal IgG2a against a recombinant fragment (GST-tag) corresponding to human GBA (aa 146-236). Reacts with human. Suitable for western blot, immunohistochemistry (paraffin and frozen), immunocytochemistry/immunofluorescence.

Western blot

WT and GBA-KO HEK 293 and A549 cells. Cell samples were lysed with PBS containing 1% SDS, 5 mM EDTA, and 1 mM sodium vanadate (Sigma catalog no. S6508).

Coupled to horseradish peroxidase.

Chemiluminescent horseradish peroxidase (HRP) substrate. Images were captured with an Alpha-Innotech Fluorchem detector.