This is a knockout-validated antibody summary, based on the publication "The PAX-SIX-EYA-DACH network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: Santa Cruz
Antibody: GATA1
Catalog number: sc-266
Summary: Rat monoclonal IgG2a against bacterially expressed murine GATA-1. Recommended for detection of GATA-1 of mouse, rat and human origin by western blot, immunoprecipitation and immunofluorescence.
Western blot
Wild-type and GATA1-KO TF1 cells.
1:200 dilution.
HRP-conjugated secondary antibodies (Jackson ImmunoResearch).
Enhanced chemiluminescence (ECL; Thermo Fisher Scientific).
Representative western blot of protein extracts generated from wild-type versus GATA1 KO TF1 cells probed with anti-HBB, anti-GATA1, anti-SIX1 and anti-Actin antibodies. Please see Figure 5c in the article [1].
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