This is a knockout-validated antibody summary, based on the publication "GABARAPL1 Inhibits EMT Signaling through SMAD-Tageted Negative Feedback", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of GABARAPL1 levels in A549c, ACHNc, and KO GABARAPL1 cells untreated or treated with 50 mM NH4Cl for 2 h. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: GABARAPL1

Catalog number: 26632

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the amino terminus of human GABARAPL1 protein. Reacts with human, mouse and rat. Suitable for western blot, immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

WT and GABARAPL1-KO A549c and ACHNc cells.

5% nonfat milk or BSA (bovine serum albumin) in Tris-buffered saline with Tween 20 (20 mM Tris-HCl, pH 7.6, 137 mM NaCl 0.1% Tween 20).

Overnight at 4 °C.

HRP-conjugated anti-rabbit (BI2413C, Diagenode).

Clarity Western ECL substrate (1705051, Bio-Rad), and chemiluminescence was monitored using a Bio-Rad ChemiDoc XRS+.