This is a knockout-validated antibody summary, based on the publication "Genetic Deletion of GABAA Receptors Reveals Distinct Requirements of Neurotransmitter Receptors for GABAergic and Glutamatergic Synapse Development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative images showed the loss of β3 subunit in hippocampal neurons expressing β1-3 gRNA vector as compared to neurons expressing the empty gRNA vector (GFP) (GFP, n = 15; β1-3 gRNAs, n = 15; ∗∗∗∗p < 0.0001, t-test; N = 3). Scale bar, 5 µm. From [1].

Mouse monoclonal IgG1

Company: Neuromab

Antibody: GABA-A-R-Beta3

Catalog number: 75-149

Summary: Mouse monoclonal IgG1 against a fusion protein corresponding to mouse GABA-A-R-Beta3 (amino acids 370-433). Reacts with mouse, rat and human. Suitable for immunoblotting, immunocytochemistry and

immunohistochemistry.

Immunocytochemistry

Control and GABA(A)R β1-3 KO mice hippocampal neurons. Cells grown on coverslips were rinsed with PBS twice and fixed in 4% paraformaldehyde (PFA)/4% sucrose/1x PBS solution for 15 min at RT or 1% PFA in 0.1 M Na-acetate buffer for 13 min at RT.

0.1% Triton X-100/10% normal goat serum in 1x PBS for 1 h.

1:1,000 dilution in 3%NGS/1x PBS incubated overnight at 4°.

Alexa Fluor 405, 555 or 647-conjugated IgG for 1 h.

Zeiss LSM 880 laser scanning confocal microscope using a 63 × oil objective (numerical aperture 1.4) at room temperature.

The same clone (N87/25) is sold as BioLegend 818501, 818503, 818504; Abcam ab98968; Neuromab 73-149, 75-149.