This is a knockout-validated antibody summary, based on the publication "Role of distinct surfaces of the G9a ankyrin repeat domain in histone and DNA methylation during embryonic stem cell self-renewal and differentiation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2A
Company: R&D Systems
Antibody: G9a
Catalog number: PP-A8620A-00
Summary: Mouse monoclonal IgG2A against recombinant mouse G9a/EHMT2 aa 15-114. Reacts with human and mouse. Suitable for western blot, immunohistochemistry,
Immunoprecipitation and ELISA.
Western blot
G9a-null mESCs
chemiluminescence using HyGLO (Denville Scientific) and Super Signal West Dura (Thermo Scientific) for proteins with low expression levels
If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.
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