This is a knockout-validated antibody summary, based on the publication "The methyltransferase G9a regulates HoxA9-dependent transcription in AML", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Efficient inactivation of the targeted G9a locus in BMMs from G9a−/− (Vav) mice. Whole-cell lysates from BMMs were analyzed by Western blot. The absence of G9a and a characteristic decrease in GLP and H3K9me2 levels were observed. H3 served as loading control. From [1].

Mouse monoclonal IgG2a

Company: R&D

Antibody: G9a

Catalog number: PP-A8620A-00

Summary: Mouse monoclonal IgG2a against recombinant mouse G9a/EHMT2 aa 15-114. Reacts with mouse and human by western blot, immunoprecipitation, immunohistochemistry and ELISA.

Western blot

Whole-cell lysates from BMMs derived from G9a+/− (Vav-Cre) and G9a−/− (Vav-Cre) mice.

Odyssey infrared system (LI-COR Biosciences).

The same clone (A8620A) is sold as Invitrogen 435200; R and D Systems PP-A8620A-00.