G3BP1 antibody | knockout validation | BD Biosciences 611126
DOI
//dx.doi.org/10.13070/ko.en.6.1856
Date
2016-11-25

This is a knockout-validated antibody summary, based on the publication "Feline Calicivirus Infection Disrupts Assembly of Cytoplasmic Stress Granules and Induces G3BP1 Cleavage", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

G3BP1 antibody | knockout validation | BD Biosciences 611126 figure 1
Figure 1. Western blot of lysates from wild-type and G3BP1 -/- HeLa-R19 cells were probed with anti-G3BP antibody. Tubulin acts as a loading control. From [1].
Antibody information

Mouse monoclonal

Company: BD Biosciences

Antibody: G3BP

Catalog number: 611126 (Clone 23/G3BP)

Summary: Mouse monoclonal antibody raised against a polypeptide corresponding to residues 210-323 of human G3BP. Supplier recommended for WB and IF. Reacts with human G3BP.

Validation Method

Western blot

Sample

Lysates of wild-type and G3BP1 -/- HeLa-R19 cells.

Primary incubation

Anti-G3BP antibody.

Secondary incubation

Peroxidase-conjugated secondary antibody (Dako).

Detection

Super Signal West chemiluminescent reagent with Fiji radiographic film.

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Humoud M, Doyle N, Royall E, Willcocks M, Sorgeloos F, van Kuppeveld F, et al. Feline Calicivirus Infection Disrupts Assembly of Cytoplasmic Stress Granules and Induces G3BP1 Cleavage. J Virol. 2016;90:6489-501 pubmed publisher