This is a knockout-validated antibody summary, based on the publication "Blunted apoptosis of erythrocytes in mice deficient in the heterotrimeric G-protein subunit Gαi2", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blots showing Gαi2 (40 kDa) and GAPDH (37 kDa) expression in erythrocytes from Gαi2+/+ (bands 1, 2 and 3) or Gαi2−/− (bands 5, 6 and 7) mice and humans (band 4) in whole blood (bands 1 and 5), diluted whole blood (bands 2 and 6; 1:3.7 dilution) and purified erythrocytes (bands 3 and 7). From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: G-protein alpha inhibitor 2

Catalog number: ab157204

Summary: Rabbit monoclonal IgG against a synthetic peptide within human G protein alpha Inhibitor 2. Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence, and flow cytometry. Unsuitable for immunoprecipitation.

Western blot

Erythrocytes from Gαi2+/+ and Gαi2−/− mouse. Ghost membranes were pelleted (15,000 g for 20 min at 4 °C) and lysed in 200 μl lysis buffer (50 mM Tris-HCl, pH 7.5; 150 mM NaCl; 1% Triton X-100; 0.5% SDS; 1 mM NaF; 1 mM Na3VO4; and 0.4% β-mercaptoethanol) containing protease inhibitor cocktail (Sigma, Schnelldorf, Germany).

5% nonfat milk in TBS-0.10% Tween 20 at room temperature for 1 h.

1:5,000 dilution at 4 °C overnight.

1:2,000 dilution anti-rabbit antibody (Cell Signaling) for 1 h at room temperature.

ECL detection reagent (Amersham, Freiburg, Germany).