This is a knockout-validated antibody summary, based on the publication "TRAF2 and OTUD7B govern a ubiquitin-dependent switch that regulates mTORC2 signalling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: GβL
Catalog number: 3274
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gln210 of human GβL. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.
Western blot
GβL+/+ and GβL−/− MEFs.
1:1,000 dilution.
1:3,000 dilution peroxidase-conjugated secondary anti-rabbit (Sigma, A4914) in 5% non-fat milk.
Immunoblot analysis of whole cell lysates derived from GβL+/+ MEFs or GβL−/− MEFs. Courtesy from the Authors.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.