This is a knockout-validated antibody summary, based on the publication "Fumarate Mediates a Chronic Proliferative Signal in Fumarate Hydratase-Inactivated Cancer Cells by Increasing Transcription and Translation of Ferritin Genes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Fumarase
Catalog number: 4567
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gly354 of human fumarase (FH). Reacts with human, mouse, rat, hamster, and monkey by western blot.
Western blot
Control and FH-knockout HEK293 cells.
1:1,000 dilution in BSA.
Western blots of HEK293 cells upon CRISPR-Cas9-mediated FH knockout. ACTB was used as a loading control. Please see Figure 2c in the article [1].
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