This is a knockout-validated antibody summary, based on the publication "mTOR inhibitors lower an intrinsic barrier to virus infection mediated by IFITM3", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. SDS-PAGE and western blot analysis of whole cell lysates derived from HeLa (WT and IFITM3 KO). Immunoblotting was performed with anti-IFITM3 and anti-IFITM2/3. Actin served as loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Fragilis

Catalog number: ab109429

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Fragilis (aa 1-100, internal sequence). Reacts with human by western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunoprecipitation.

Western blot

WT and IFITM3 KO HeLa cells. Whole cell lysis was performed with radioimmunoprecipitation assay (RIPA) buffer (Thermo Fisher) supplemented with Halt Protease Inhibitor mixture EDTA-free (Thermo Fisher).

Odyssey blocking buffer in PBS (Li-Cor).

Secondary antibodies conjugated to DyLight 800 or 680 (Li-Cor).

Li-Cor Odyssey imaging system.

The same clone (EPR5242) is sold as LifeSpan Biosciences LS-B7510; Abcam ab109429, ab198573.