This is a knockout-validated antibody summary, based on the publication "Reduced mitochondrial fusion and Huntingtin levels contribute to impaired dendritic maturation and behavioral deficits in Fmr1-mutant mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1k
Company: Millipore
Antibody: Fragile X Mental Retardation Protein
Catalog number: MAB2160
Summary: Mouse monoclonal IgG1k against a fusion protein with a full length human FMRP. Reacts with human, mouse and rat. Suitable for western blot, immunocytochemistry, immunohistochemistry and ELISA. Not recommended for flow cytometry or immunoprecipitation.
Immunohistochemistry
Brain sections from control and conditional FMRP knockout mice. Brains were dissected out, post-fixed overnight in 4% PFA and frozen sections were processed.
TBS++ (TBS containing 3% goat or donkey serum and 0.2% Triton X-100) for 1 h at room temperature.
1:500 dilution in TBS++ overnight in 4 ℃.
1:500 dilution anti-mouse 647 (Invitrogen) for 1 h at room temperature.
Nikon A1 confocal microscope.
Representative confocal images (from 3 independently repeated experiments with similar results) of FMRP (green), DCX (white) staining of brain sections from Ctrl;Cre;tdT (left) and cKO;Cre;tdT (right) mice. Scale bars, 20 μm. Please see Figure 1d in the article [1].
The same clone (1C3) is sold as LifeSpan Biosciences LS-C15265; EMD Millipore MAB2160.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.