This is a knockout-validated antibody summary, based on the publication "Metabolic inflexibility impairs insulin secretion and results in MODY-like diabetes in triple FoxO-deficient mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Foxo3

Catalog number: 2497

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Glu50 of human FoxO3a. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation and immunofluorescence/immunocytochemistry.

Western blot

Islet extracts of 24-week-old db/db, WT (C57BL/6) and FoxO-KO.

Please see Figure 1b in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.