This is a knockout-validated antibody summary, based on the publications "Regulation of autophagy and the ubiquitin-proteasome system by the FoxO transcriptional network during muscle atrophy", as cited below [1], "Genetic Ablation of FOXO in Mice to Investigate Its Physiological Role" (immunohistochemistry, figure 3) [2], and "Disruption of FOXO3a-miRNA feedback inhibition of IGF2/IGF-1R/IRS1 signaling confers Herceptin resistance in HER2-positive breast cancer" for western blot knockout validation (figure 2d, s2e) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal
Company: Cell Signaling Technology
Antibody: FoxO3
Catalog number: 12829 (Clone D19A7)(from kit 9946)
Summary: Rabbit monoclonal antibody raised against a recombinant protein corresponding to the C-terminal region of human FoxO3. Supplier recommended for WB, IP, IHC, IF and flow cytometry. Reacts with human, mouse and rat FoxO3.
Western blot; The antibody is also knockout-validated from immunohistochemistry (see figure 3 in [2] ).
Skeletal muscle lysates from wild-type mice and mice with muscle-specific triple knockout of FoxO1, 3 and 4.
Samples contain small numbers of contaminating endothelial, fibroblasts, macrophages and blood cells which will not be deleted for FoxO1, 3, 4 and will likely give rise to a background signal.
Anti-FoxO3 diluted 1:1000.
Super Signal West Pico ECL reagent (Pierce).
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