This is a knockout-validated antibody summary, based on the publication "A mutant allele encoding DNA binding-deficient FoxO1 differentially regulates hepatic glucose and lipid metabolism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling Technology

Antibody: FoxO1

Catalog number: 2880

Summary: Rabbit monoclonal IgG against a fusion protein corresponding to carboxy-terminal residues of human FoxO1. Reacts with human, mouse, rat and monkey. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

Livers from control, FoxO1−/− and FoxO1−/dbd mice. Frozen livers were homogenized in ice-cold lysis buffer (20 mmol/L Tris-HCl, 150 mmol/L NaCl, 10% glycerol, 2% NP-40, 1 mmol/L EDTA, 20 mmol/L NaF, 30 mmol/L Na4P2O7, 0.2% [w/v] SDS, and 0.5% [w/v] sodium deoxycholate) supplemented with protease/phosphatase inhibitors (Cell Signaling Technology).

Western blot of liver extracts from fasted control, FoxO1−/− and FoxO1−/dbd mice. Please see Figure 1e in the article [1].