This is a knockout-validated antibody summary, based on the publication "Transcriptomic and CRISPR/Cas9 technologies reveal FOXA2 as a tumor suppressor gene in pancreatic cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Company: Cell Signaling Technology

Antibody: FoxA2

Catalog number: 8186

Summary: The immunogen is a synthetic peptide corresponding to residues surrounding Gly138 of human FoxA2/HNF3β protein. The antibody recognizes endogenous levels of total FoxA2/HNF3β protein. It is reactive against human, mouse, and rat, and recommended for western blotting (1:1000), immunoprecipitation (1:50), and immunocytochemistry (1:400).

Western blot

Human pancreatic cancer cell line PANC-1 and its FOXA2-null derivative line.

Blocked with 5% nonfat dry milk in PBS and 0.05% Tween 20 and probed with the antibody at the dilution (1:1,000)

HRP-labeled secondary antibodies (1:1,000).

Enhanced chemiluminescence (ECL) reagent (T) and exposed in Eastman Kodak 440 Image Station.

The article mistakenly lists the catalog number as 8189.

Please see Figure 6b in the article [1].