Fbw7 antibody | knockout validation | Bethyl Laboratories A310-720A

DOI

//dx.doi.org/10.13070/ko.en.6.1924

Date

2016-12-12

This is a knockout-validated antibody summary, based on the publication " Isoform-specific SCF(Fbw7) ubiquitination mediates differential regulation of PGC-1α", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit polyclonal

Company: Bethyl Laboratories

Antibody: Fbw7

Catalog number: noted wrongly as A310-720A in the article, should be A301-720A

Summary: Rabbit polyclonal antibody. Raised against residues 1-50 of human Fbw7.

Supplier recommended for WB and IP.

Reacts with human Fbw7.

Validation Method

Western blot

Sample

Lysates of wild-type and Fbw7 knockout HCT116 cells.

Primary incubation

Anti-Fbw7 antibody.

Secondary incubation

HRP-conjugated secondary antibody.

Detection

ECL reagent (GE Healthcare).

Figure

See Supplementary Figure S1 in [1].

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References

Trausch-Azar J, Abed M, Orian A, Schwartz A. Isoform-specific SCF(Fbw7) ubiquitination mediates differential regulation of PGC-1α. J Cell Physiol. 2015;230:842-52 pubmed publisher

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