This is a knockout-validated antibody summary, based on the publications "Essentiality of Fatty Acid Synthase in the 2D to Anchorage-Independent Growth Transition in Transforming Cells", as cited below [1], "Cholesterol biosynthesis supports the growth of hepatocarcinoma lesions depleted of fatty acid synthase in mice and humans" for immunohistochemistry knockout validation (see [2] figure 1c), and "Pharmacological inhibition of fatty acid synthesis blocks SARS-CoV-2 replication" for western blot knockout validation (figure 1e) [3]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative images of sections of the mammary pad of FASN+/+; PyMT and FASN∆/∆; PyMT animals stained for FASN. The areas negative for FASN have normal or at most proliferative (but not invasive) ducts (asterisks). Invasive growth was only observed in the FASN-positive areas of the FASN∆/∆; PyMT mice (arrows). Scale bars, 200 μm (low magnification) and 100 μm (high magnification; i, ii, iii). From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Fatty Acid Synthase

Catalog number: 3180

Summary: Rabbit monoclonal IgG against a synthetic peptide around Gly46 corresponding to the sequence of human fatty acid synthase. Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation, immunohistochemistry (paraffin and frozen), immunofluorescence/immunocytochemistry.

Immunohistochemistry

Control FASN+/+ and FASN-KO mouse mammary pads. Tissues were fixed in 10% buffered formalin (Sigma) and embedded in paraffin.

1:50 dilution.

Appropriate biotinylated secondary Ab.

Incubation with streptavidin–horseradish peroxidase and diaminobenzidine system. Slides were digitized and analyzed by using the ZEISS Zen 2.3 Imaging Software (Zeiss).