Fatty Acid Synthase antibody | knockout validation | Abcam ab128870

This is a knockout-validated antibody summary, based on the publication "Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Fatty Acid Synthase

Catalog number: ab128870

Summary: Rabbit monoclonal antibody against a synthetic peptide within human Fatty Acid Synthase (aa 2,450-2,550; C terminal). Reacts with human and it is predicted to work with mouse and rat. Suitable for western blot, immunohistochemistry (paraffin), immunocytochemistry/immunofluorescence and flow cytometry (intra).

Validation Method

Western blot

Sample

Parental wildtype (WT) and FASN-KO HAP1 cells.

Primary incubation

1:2,000 dilution.

Detection

X-ray film using Super Signal chemiluminescence reagent (Thermo Scientific) and Western Lightning ECL Pro (PerkinElmer).

Figure : Western blot depicting FASN and β-Actin levels in HAP1 parental wildtype (WT) and FASN-KO cells. Representative data from three biologically independent experiments. Please see Extended Data Figure 1 in the article [1].
References
  1. Aregger M, Lawson K, Billmann M, Costanzo M, Tong A, Chan K, et al. Systematic mapping of genetic interactions for de novo fatty acid synthesis identifies C12orf49 as a regulator of lipid metabolism. Nat Metab. 2020;2:499-513 pubmed publisher