Fatso antibody | knockout validation

Fatso antibody | knockout validation | Santa Cruz sc-271713

This is a knockout-validated antibody summary, based on the publication "Autophagy of the m 6 A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis confirming FTO knockout (KO) in As-T cells using two independent sgRNAs using CRISPR. From [1].

Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: Fatso

Catalog number: sc-271713

Summary: Mouse monoclonal antibody against amino acids 252-505 mapping at the C-terminus of human Fatso (FTO). Recommended for detection of Fatso of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

WT and FTO knockout As-T cells.

Primary incubation

1:500 dilution.

Secondary incubation

Rabbit Anti-Mouse IgG (Cell Signaling Technology, 58802).

References

Cui Y, Yang S, Wei J, Shea C, Zhong W, Wang F, et al. Autophagy of the m6A mRNA demethylase FTO is impaired by low-level arsenic exposure to promote tumorigenesis. Nat Commun. 2021;12:2183 pubmed publisher

contribute

If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.