This is a knockout-validated antibody summary, based on the publication "The Fas/Fap-1/Cav-1 complex regulates IL-1RA secretion in mesenchymal stem cells to accelerate wound healing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a
Company: Millipore
Antibody: Fas
Catalog number: 05-351
Summary: Rat monoclonal IgG2a against a recombinant protein containing the cytoplasmic domain of mouse Fas. Reacts with mouse and bovine. Suitable for western blot and immunoprecipitation.
Western blot
Gingiva-derived Mesenchymal stem cells (GMSCs). Cells were lysed in M-PER mammalian protein extraction reagent (Thermo Fisher Scientific) with protease and phosphatase inhibitors (Roche).
5% nonfat dry milk and 0.1% Tween 20 for 1 hour.
Diluted in blocking solution overnight.
1:10,000 dilution horseradish peroxidase–conjugated secondary antibody (Santa Cruz Biotechnology in blocking solution for 1h at room temperature.
SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher Scientific) and Biomax film (Kodak).
Western blotting of the indicated proteins from WT control and Fas-deficient MRL/lpr GMSCs (n = 3). Please see Figure 2e in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.