This is a knockout-validated antibody summary, based on the publication "APCFZR1 prevents nondisjunction in mouse oocytes by controlling meiotic spindle assembly timing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Representative immunoblots of GV-arrested oocytes collected from Fzr1−/− mice or control littermates. GAPDH served as loading control. At least three separate runs were performed, with n = 50 oocytes per lane. From [1].

Mouse monoclonal IgG1

Company: Abcam

Antibody: FZR1

Catalog number: ab3242

Summary: Mouse monoclonal IgG1 against recombinant full length human FZR1 protein. Reacts with mouse and human. Suitable for western blot, immunoprecipitation, and flow cytometry.

Western blot

Oocytes from Fzr1−/− mice or control littermates. Oocytes were lysed in reducing SDS buffer.

Horseradish peroxidase (Dako, Glostrup, Denmark).

ECL Detection reagents (GE Healthcare Australia, Rydalmere, Australia).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.