This is a knockout-validated antibody summary, based on the publication "Ablation of liver Fxr results in an increased colonic mucus barrier in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a
Company: Invitrogen
Antibody: FXR
Catalog number: 417200
Summary: Mouse monoclonal antibody against recombinant human FXR (aa 2-126 ). Reacts with human, mouse and rat. Suitable for western blot, immunohistochemistry, immunocytochemistry/immunofluorescence, immunoprecipitation and ELISA.
Western blot
Control and Fxr-KO mouse tissues.
Overnight at 4 °C
1:10,000 dilution peroxidase-conjugated rabbit anti mouse antibody (Agilent Dako, P0260) for 1h at room temperature.
Enhanced chemiluminescence detection system.
The same clone (A9033A) is sold as R&D Systems PP-A9033A-00; Invitrogen 417200.
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.