This is a knockout-validated antibody summary, based on the publication "Structural, super-resolution microscopy analysis of paraspeckle nuclear body organization", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Confirmation of the specificity of monoclonal antibody against Fus. Mixtures of MEFs derived from WT and KO mice of Fus were stained. Note the complete absence of signals in the Fus KO MEFs (arrowheads). From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: FUS/TLS

Catalog number: sc-47711

Summary: Mouse monoclonal IgG1 (kappa light chain) against a fusion protein corresponding to the C-terminus of human FUS/TLS. Recommended for detection of FUS/TLS of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Immunocytochemistry

MEFs prepared from wild-type (WT) and Fus-KO mice embryos.

Fig 5E may have used rabbit polyclonal antibody against Fus (ab84078; Abcam).