FUS/TLS antibody | knockout validation | Abcam ab70381
DOI
//dx.doi.org/10.13070/ko.en.6.1703
Date
2016-10-07

This is a knockout-validated antibody summary, based on the publication "Spliceosome integrity is defective in the motor neuron diseases ALS and SMA", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

FUS/TLS antibody | knockout validation | Abcam ab70381 figure 1
Figure 1. DIV 21 hippocampal neurons form FUS−/− mice or littermates were stained for SMN to analyze the requirement of FUS/TLS for Gem formation. Bars: 10 µm. From [1].
Antibody information

Rabbit polyclonal

Company: Abcam

Antibody: FUS/TLS

Catalog number: ab70381

Summary: Rabbit polyclonal against a region between residues 400 and 450 of human TLS/FUS. Reacts with mouse and human and is predicted to work with rat, rabbit, cow, dog, pig, and monkey (among others).

Suitable for: western blot, immunoprecipitation, immunocytochemistry, immunohistochemistry-paraffin.

Validation Method

Immunocytochemistry

Sample

Hippocampal neurons isolated from E16.5 C57BL6 or FUS−/− mouse embryos

Blocking agent

1% normal goat serum

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Tsuiji H, Iguchi Y, Furuya A, Kataoka A, Hatsuta H, Atsuta N, et al. Spliceosome integrity is defective in the motor neuron diseases ALS and SMA. EMBO Mol Med. 2013;5:221-34 pubmed publisher