FOXC1 antibody | knockout validation | Abcam ab227977

This is a knockout-validated antibody summary, based on the publication "FOXC1 Negatively Regulates DKK1 Expression to Promote Gastric Cancer Cell Proliferation Through Activation of Wnt Signaling Pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

FOXC1 antibody | knockout validation | Abcam ab227977 figure 1
Figure 1. FOXC1 knockout and overexpression efficiency was confirmed using western blotting, and GAPDH was used as an internal control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: FOXC1

Catalog number: ab227977

Summary: Rabbit monoclonal antibody against a synthetic peptide of FOXC1. Reacts with mouse and human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin and frozen) and flow cytometry (intra).

Validation Method

Western blot

Sample

Control and FOXC1 AGS and MKN-45 cells.

Secondary incubation

Anti-rabbit IgG (Proteintech).

References
  1. Jiang J, Li J, Yao W, Wang W, Shi B, Yuan F, et al. FOXC1 Negatively Regulates DKK1 Expression to Promote Gastric Cancer Cell Proliferation Through Activation of Wnt Signaling Pathway. Front Cell Dev Biol. 2021;9:662624 pubmed publisher