This is a knockout-validated antibody summary, based on the publication "Neuroligin 1, 2, and 3 Regulation at the Synapse: FMRP-Dependent Translation and Activity-Induced Proteolytic Cleavage", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of immunoprecipitated FMRP from mouse synaptoneurosomes shows FMRP in input and FMRP IP from WT mice. Fmr1 KO extracts and IgG IPs were used as controls. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: FMRP

Catalog number: 7104S

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Gly552 of human FMRP. Reacts with human,mouse, rat, and monkey. Suitable for western blot and immunofluorescence (immunocytochemistry).

Western blot

Synaptoneurosomes from hippocampi and a part of adjacent cerebral cortex of WT and Fmr1 KO mice. Tissues were lysed in 10 mM HEPES, pH 7.4, 200 mM NaCl, 30 mM EDTA, 0.5% Triton X-100 with protease inhibitor cocktail cOmplete (Roche, Indianapolis, IN) and 80 U/ml RiboLock RNase Inhibitor (Thermo Scientific).

5% BSA and 3% normal goat serum (NGS) in PBST for 1 h at RT.

1:1,000 dilution overnight at 4 °C with gentle shaking.

1:10,000 dilution peroxidase-labeled secondary antibodies (Vector Laboratories, Burlingame, CA) in 5% BSA in PBST for 1 h at RT.

Enhanced chemiluminescence using Amersham ECL Prime Western Blotting Detection Reagent (GE Healthcare) and Amersham Imager 600 (GE Healthcare). S