FMO5 antibody | knockout validation | Proteintech 13699-1-AP
DOI
//dx.doi.org/10.13070/ko.en.7.1941
Date
2017-01-13

This is a knockout-validated antibody summary, based on the publication "The phenotype of a knockout mouse identifies flavin-containing monooxygenase 5 (FMO5) as a regulator of metabolic ageing", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

FMO5 antibody | knockout validation | Proteintech 13699-1-AP figure 1
Figure 1. From Figure 1 in [1]. Western blot of liver lysates from wild-type and FMO5 knockout mice probed with anti-FMO5 antibody.
Antibody information

Rabbit polyclonal

Company: Proteintech

Antibody: FMO5

Catalog number: 13699-1-AP

Summary: Rabbit polyclonal antibody raised against a fusion protein containing residues 34-285 of human FMO5.

Supplier recommended for WB, IP, IHC and elisa.

Antigen affinity-purified.

Reacts with human, mouse and rat FMO5.

Validation Method

Western blot

Sample

Liver lysates from wild-type and FMO5 knockout mice.

Primary incubation

Anti-FMO5 antibody.

Secondary incubation

HRP-conjugated donkey anti-rabbit IgG (Abcam).

Detection

ECL reagent in conjunction with LAS 1000 image reader (Fuji).

Disclaimer

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

References
  1. Gonzalez Malagon S, Melidoni A, Hernandez D, Omar B, Houseman L, Veeravalli S, et al. The phenotype of a knockout mouse identifies flavin-containing monooxygenase 5 (FMO5) as a regulator of metabolic ageing. Biochem Pharmacol. 2015;96:267-77 pubmed publisher