This is a knockout-validated antibody summary, based on the publication "Formin-like 1 mediates effector T cell trafficking to inflammatory sites to enable T cell-mediated autoimmunity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. After 5 min of transendothelial migration (TEM) under flow cells were fixed, permeabilized, and stained with a mouse monoclonal anti-FMNL1 antibody followed by secondary staining with a Dylight-649 conjugated Donkey anti-mouse secondary antibody. Representative images of a WT (top) or FMNL1 KO T cell (bottom). Images are maximum Z projections of the indicated channel and a composite overlay image. From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: FMNL1

Catalog number: sc-390466

Summary: Mouse monoclonal IgG1 κ against amino acids 161-220 mapping within an internal region of human FMNL1. Recommended for detection of FMNL1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Immunocytochemistry

WT and FMNL1 KO T cells from mice.

0.5% (w/v) saponin (Millipore-Sigma) 2% (v/v) FBS (Corning) 2% (v/v) normal donkey serum (Jackson ImmunoResearch Laboratories) with 0.05% (w/v) sodium azide (Millipore Sigma) in PBS (Millipore-Sigma) for 1 hr at room temperature.

1:100 dilution in blocking buffer overnight at 4°C.

1:100 dilution Dylight-649 conjugated Donkey anti-mouse secondary antibody (Jackson ImmunoResearch) in saponin buffer for 1 hr at room temperature.

3i (Intelligent Imaging Innovations) Marianas spinning-disk confocal microscope system equipped with a Zeiss inverted stand and a Yokogawa spinning disk unit.