This is a knockout-validated antibody summary, based on the publication "RRRRR", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Generation of WIBR3 5iLA FLCN KO and Elf1 2iL-I-F FLCN KO line, and overexpression of FLCN-GFP fusion protein in wild type and FLCN KO background. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: 3697

Catalog number: FLCN

Summary: Rabbit monoclonal IgG against recombinant human FLCN protein. Reacts with human by western blot and immunoprecipitation.

Western blot

Control and FLCN-KO WIBR3 and Elf1 cell lines. Cells were lysed directly on the plate with a lysis buffer containing 20 mM Tris-HCl pH 7.5, 150 mM NaCl, 15% glycerol, 1% Triton x-100, 1 M ß-glycerolphosphate, 0.5 M NaF, 0.1 M sodium pyrophosphate, orthovanadate, PMSF, and 2% sodium dodecyl sulfate (SDS).

5% milk for 1 h.

1:2,000 dilution overnight at 4 °C.

1:10,000, goat anti-rabbit HRP conjugate(Bio-Rad) for 1 h.

Immobilon-luminol reagent assay (EMD Millipore).