This is a knockout-validated antibody summary, based on the publications "The tumor suppressor FLCN mediates an alternate mTOR pathway to regulate browning of adipose tissue" (shown below) [1] and "Myeloid Folliculin balances mTOR activation to maintain innate immunity homeostasis" (see figure 1a, western blot) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot showing the efficiency of FLCN protein deletion in BAT and iWAT in FLCN adipKO compared to WT. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: FLCN

Catalog number: ab124885

Summary: Rabbit monoclonal IgG against a recombinant tagged protein fragment corresponding to human FLCN. Reacts with mouse, rat, and human by western blot. Unsuitable for flow cytometry ,immunocytochemistry, immunohistochemistry (paraffin) or immunoprecipitation.

Western blot

BAT and iWAT from WT and adipose tissue-specific deletion of FLCN. Tissue or cell samples were lysed by RIPA buffer containing phosphatase inhibitor (PhosSTOP, Roche) and proteinase inhibitor (Complete miniproteinase inhibitor cocktail, Roche).

5% skim milk for 1 h.

HRP-conjugated secondary antibodies.

Digital imager (GE Healthcare Life Sciences, ImageQuant LAS 4000).