This is a knockout-validated antibody summary, based on the publication "Genetic dissection of Flaviviridae host factors through genome-scale CRISPR screens", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: FLAD1

Catalog number: sc-376819

Summary: Mouse monoclonal IgG1 (kappa light chain) against an epitope mapping between amino acids 157-195 within an internal region of FLAD1 of human origin. Recommended for detection of FLAD1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

WT and CRISPR-induced Flad1-KO Huh7.5.1 cells. Cell pellets were lysed using Laemmli SDS sample buffer containing 5% beta-mercaptoethanol.

5% non-fat milk, 0.1% Tween-20 PBS .

1:250 dilution in blocking buffer overnight at 4 C rotating.

1:5,000 dilution HRP-conjugated secondary anti-mouse antibodies (Genetex) for 1 hr at room temperature.

West Pico and Extended Duration peroxide solutions (Pierce) and visualized on film.

Immunoblot of CRISPR-induced FLAD1 KO cells. p84 was used as loading control. Please see Extended Data Figure 7b in the article [1].

RPS25 (Abcam, 102940), MAGT1 (Proteintech, 17430-1-AP), RPN1 (gift from Martin Ivessa), STT3B (Sigma, HPA036646), ELAVL1 (Santa Cruz, sc-5261), RFK (Sigma, SAB1409492) and SRRD (Sigma, HPA002945) are also KO validated (WB), although some of them are polyclonals.